Miki Ii

Assistant Professor

University of Alaska Anchorage
3101 Science Circle
Integrated Science Building  201P
Anchorage, Alaska 99508
   (IDeA Network of Biomedical Research Excellence)
University of Alaska Anchorage
   Department of Biological Sciences


● DNA Repair & Genome Stability
● Posttranslational protein modification (SUMOylation)   
● Proteins related to cancer & aging
● B.S. 1992 Biology
   Kagoshima University, Kagoshima, Japan
M.S. 1994 Biology
   Kagoshima University, Kagoshima, Japan
● Ph.D. 2002 Molecular Biology
   Kyushu University, Fukuoka, Japan
● Post Doctoral 2003 Molecular Biology
   Kansas State University, Manhattan, KS
Post Doctoral 2007 Genetics & Molecular Biology
   Rutgers University, Piscataway, NJ
● Post Doctoral 2007 Molecular Biology
   Kwansei Gakuin University, Hyogo, Japan
Research Overview
Maintenance of genome stability is indispensable for health of the cells.  Accumulation of DNA damage results in mutations and DNA double-strand breaks that are referred to genome instability unless the DNA damage is repaired. Loss of or defects in proper DNA repair system causes premature aging, cancers and a variety of genetic diseases. To contribute to human health care, our research is focused on study of DNA repair pathways that are highly conserved in humans and are responsible for genome stability to avoid cancer formation and accelerated aging. Another focus is to define how DNA repair proteins are regulated by protein SUMOylation. We use the budding yeast Saccharomyces cerevisiae as a model system.   
Current Research Projects
Maintenance of genome stability mediated by homologous recombination and related 
   DNA repair enzymes in yeast
Regulation of DNA repair proteins by protein SUMOylation
Peer Reviewed Publications (in chronological order)
1. Takeshi Sekiguchi, Eiji Hirose, Nobutaka Nakashima, Miki Ii, and Takeharu Nishimoto  (2001). Novel G proteins, Rag C and Rag D, interact with GTP-binding proteins, Rag A and Rag B. J. Biol. Chem., 276, 7246-7257. [PMID:11073942]
2. Miki Ii and Katsuyoshi Mihara (2001). Insertion of mitochondrial DNA-encoded F1F0-ATPase subunit 8 across the mitochondrial inner membrane in vitro. J. Biol. Chem., 276, 24704- 24712. PMID:11320097]
3. Hui He*, Tobias Von Der Haar*, C. Ranjit Singh*, Miki Ii*, Bin Li, Alan G. Hinnebusch, John E.G. McCarthy, and Katsura Asano (2003). The yeast eukaryotic initiation factor 4G (eIF4G) HEAT domain interacts with eIF1 and eIF5 and is involved in stringent AUG selection. Mol. Cell. Biol., 23, 5431-5445. (*:Authors contributed equally) [P MCID: PMC165723]
4. C. Ranjit Singh*, Hui He*, Miki Ii, Yasufumi Yamamoto, and Katsura Asano (2004). Efficient  incorporation of eukaryotic initiation factor 1 into the multifactor complex is critical for  formation of  functional ribosomal preinitiation complex in vivo. J. Biol. Chem., 279, 31910-31920. (*: Authors contributed equally) [PMID: 15145951] 
5. Miki Ii and Steven J. Brill (2005). Roles of SGS1, MUS81, and RAD51 in the repair of  lagging  strand replication defects in Saccharomyces cerevisiae. Curr. Genet., 48, 213-225. [ PMCID: PMC1828632]
6. Tania M. Roberts, Michael S. Kobor, Suzanne A. Bastin-Shanower, Miki Ii, Sonja A. Horte,  Jennifer W. Gin, Andrew Emili, Jasper Rine, Steven J. Brill, and Grant W. Brown (2006). Slx4 regulates DNA damage checkpoint dependent phosphorylation of the BRCT domain protein Rtt107/Esc4. Mol. Biol. Cell 17, 539-48. [ PMCID: PMC1345688]
7. Miki Ii‡, Tatsuya Ii, and Steven J. Brill (2007). Mus81 functions in the quality control of replication forks at the rDNA and is involved in the maintenance of rDNA repeat number in     Saccharomyces cerevisiae. Mutat. Res., 625, 1-19. (‡:Corresponding Author.) [PMCID:PMC2100401]
8. Mikhail Reibarkh, Yasufumi Yamamoto, C. Ranjit Singh, Federico del Rio, Amr Fahmy,  Bumjun Lee, Rafael E. Luna, Miki Ii, Gerhard Wagner, and Katsura Asano (2008). Eukaryotic Initiation factor (eIF) 1 carries two distinct eIF5-binding faces important for multifactor assembly and AUG selection. J. Biol. Chem., 283, 1094-103 [ PMID: 17974565]
9. Ryosuke Watanabe, Marcelo J. Murai, C. Ranjit Singh, Stephanie Fox, Miki Ii, and Katsura Asano (2010). The eukaryotic initiation factor (eIF) 4G heat domain promotes translation re-initiation in yeast both dependent on and independent of eIF4A mRNA helicase. J. Biol. Chem., 285, 21922-21933. [ PMCID: PMC2903371]
10. Dongyi Xu, Parameswary Muniandy, Elisabetta Leo, Jinhu Yin, Saravanabhavan Thangavel,  Xi Shen, Miki Ii, Keli Agama, Rong Guo, David Fox 3rd, Amom Ruhikanta Meetei, Lauren Wilson, Huy Nguyen, Nan-ping Weng, Steven J. Brill, Lei Li, Alessandro Vindigni, Yves    Pommier, Michael Seidman, and Weidong Wang (2010). Rif1 provides a new DNA binding   interface for the Bloom syndrome complex to maintain normal replication. EMBO J. 29, 3140-3155 [ PMCID: PMC2944062]
11. Naoki Nemoto, C. Ranjit Singh, Tsuyoshi Udagawa, Suzhi Wang, Elizabeth Thorson,  Zachery Winter, Takahiro Ohira, Miki Ii, Leoš Valásek, Susan J. Brown, and Katsura Asano (2010). Yeast 18S rRNA is directly involved in the ribosmeal response to stringent AUG selection during translation initiation. J. Biol. Chem. 285, 32200-32212[PMCID:PMC2952221]
12. Miki Ii§, Tatsuya Ii, Larisa I. Mironova and Steven J. Brill (2011). Epistasis analysis between homologous recombination genes in Saccharomyces cerevisiae identifies multiple repair pathways for Sgs1, Mus81-Mms4 and RNase H2. Mutat. Res. 714, 33-43. (§: Corresponding Author.) [PMID:21741981]
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