Jason Burkhead

Assistant Professor

University of Alaska Anchorage
3211 Providence
201M Integrated Science Building
Anchorage, Alaska 99508

● Biochemistry   ● Cell Biology

   (IDeA Network of Biomedical Research Excellence)
● University of Alaska Anchorage
   Department of Biological Sciences
● Molecular Biology of copper in mammalian nuclei
● Biochemistry & Cell Biology
B.S. 1995 Biology
    Indiana University, Bloomington, IN
● M.S. 2001 Biology/Botany
   Colorado State University, Ft. Collins, CO
● Ph.D. 2003 Biology / Botany
   Colorado State University, Ft. Collins, CO
● Post Doctoral 2009 Biochemistry
   Oregon Health & Science University, Portland, OR
Research Overview
My research studies molecular mechanisms of mammalian copper homeostasis. Copper is an essential micronutrient and redox active metal. Misbalance of copper metabolism can influence liver disease, exhibit neurological damage and lipid metabolism. Therefore, cellular and organismal levels of copper must be tightly maintained and the copper ion must be correctly targeted. The active research in my lab utilizes biochemistry and molecular biology, cell biology, and rodent models to gain mechanistic data on copper metabolism and its roles in health and disease. One key project investigates mechanisms and roles of the Copper Metabolism Murr1 Domain 1 (COMMD1) protein in hepatocellular copper export, including interaction of the protein with signaling lipids. A second line of study takes a systems approach to understanding mammalian copper homeostasis through study of healthy and diseased rodent models.
Current Research Projects
Phosphatidylinositol interactions with COMMD1
Mechanism of COMMD1 action in hepatocellular copper export
Systems approach to Wilson’s disease
Molecular responses to copper toxicity and deficiency in a rodent model
Peer Reviewed Publications (in chronolgical order)
1. Pilon-Smits EAH, Garifullina GF, Abdel-Ghany SE, Kato S-I, Mihara H, Hale KL, Burkhead JL, Esaki N, Kurihara T, Pilon M Characterization of a NifS-like Chloroplast Protein from Arabidopsis thaliana - Implications for Its Role in Sulfur and Selenium Metabolism. (2002) Plant Physiol. 130: 1309-1318. [PMC166651]
2. Bennett LE, Burkhead JL, Hale KL, Terry N, Pilon M, Pilon-Smits EAH Analysis of Transgenic Indian Mustard Plants for Phytoremediation of Metal-Contaminated Mine Tailings. (2003) Journal of Environmental Quality, 32: 432-440.
3. Burkhead JL , Abdel-Ghany SE, Morrill JM, Pilon-Smits EA, Pilon M. The Arabidopsis thaliana CUTA gene encodes an evolutionarily conserved copper binding chloroplast protein. Plant J. (2003) Jun;34(6):856-67 [PMID: 12795705]
4. Wangeline, AL, Burkhead, JL, Hale, KL, Lindblom, SD, Terry, N, Pilon, M, Pilon Smits, EAH Over expression of ATP Sulfurylase in Brassica juncea: Effects on Tolerance and Accumulation of Twelve Metals. (2004) Journal of Environmental Quality, 33, 54-60. 
5. Abdel-Ghany SE, Burkhead JL, Gogolin KA, Andrés-Colás N, Bodecker JR, Puig S, Peñarrubia L, Pilon M. AtCCS is a functional homolog of the yeast copper chaperone Ccs1/Lys7. FEBS Lett. (2005) Apr 25;579(11):2307-12. [PMID: 15848163]
6. Dominik Huster, Milton J. Finegold, Clinton T. Morgan, Jason L. Burkhead, Randal Nixon, Scott M. Vanderwerf, Conrad T. Gilliam, and Svetlana Lutsenko. Consequences of copper accumulation in the livers of the ATP7B-/- (Wilson Disease gene) Knock-out mice. (2006)  Am.J. Pathology; 168: 423-434 [PMC1606493]
7. Dominik Huster*, Tina D. Purnat*, Jason L. Burkhead, Martina Ralle, Oliver Fiehn, Franziska Stuckert, N. Erik Olson, Daniel Teupser, and Svetlana Lutsenko (*authors contributed equally). High Copper Selectively Alters Lipid Metabolism and Cell Cycle Machinery in the Mouse Model of Wilson Disease (2007) J. Biol. Chem. 282(11): 8343-8355. [PMID: 17205981]
8. Sergi Puig, Helena Mira, Eavan Dorcey, Vicente Sancenón, Nuria Andrés-Colás, Antoni Garcia Molina, Jason L. Burkhead, Kathryn A. Gogolin, Salah E. Abdel Ghany, Dennis J. Thiele, Joseph R. Ecker, Marinus Pilon and Lola Peñarrubia. Higher plants possess two different types of ATX1-like copper chaperones. (2007) Biochem. Biophys. Res. Comm. 354(2): 385-390. [PMID: 17223078]
9. Svetlana Lutsenko, Arnab Gupta, Jason Burkhead and Vesna Zuzel. Cellular Multitasking: The dual role of human Cu-ATPases in cofactor delivery and intracellular copper balance. (2008) Archives of Biochem. Biophys. Aug 1;476(1):22 32. [NIHMS61450]
10. Jason L. Burkhead *, Clinton T. Morgan*, Ujwal Shinde, Gabrielle Haddock, Svetlana Lutsenko. COMMD1 Forms Oligomeric Complexes Targeted to the Endocytic Membranes via Specific Interactions With Ptdins(4,5)P2.(*Authors contributed equally). (2009) Jan 2; 284(1): 696-707. [PMC2610505]
11. Jason L. Burkhead , Kathryn A. Gogolin Reynolds, Salah E. Abdel-Ghany,Christopher M. Cohu, and Marinus Pilon Copper homeostasis in plant cells. (2009) Invited Tansley Review, New Phytologist. June.182, 4. 799-816. [PMID: 19402880]
12. Martina Ralle*, Dominik Huster *, Stefan Vogt, Wiebke Schirrmeister, Jason L. Burkhead, Tony R. Capps, Lawrence Gray, Barry Lai, Edward Maryon, and Svetlana Lutsenko. Wilson's disease at a single cell level: intracellular copper trafficking activates compartment-specific responses in hepatocytes. (2010) J. Biol. Chem. Oct 1; 285(40): 30875-83 [PMID: 20647314]
13. Jason L. Burkhead, Martina Ralle, Phillip Wilmarth, Larry David and Svetlana Lutsenko.  Elevated Copper Remodels Hepatic RNA Processing Machinery In TheMouse Model Of Wilson’s Disease. (2011) J Mol Biol . 2011, Feb 11; 406(1):44-58 [PMID: 21146535]
14. Jason L. Burkhead, Lawrence Gray, and Svetlana Lutsenko. Systems Biology Approach to Wilson’s Disease. Biometals. Epub March 5, (2011). [PMID 21380607]
15.  Phillip A. Wilmarth, Kristopher K. Short, Oliver Fiehn, Svetlana Lutsenko, Larry L. David and Jason L. Burkhead,  A systems approach implicates nuclear receptor targeting in the Atp7b−/− mouse model of Wilson’s disease Metallomics , 2012, Advance Article  DOI:  10.1039/C2MT20017A
16.  Wilmarth PA, Short KK, Fiehn O, Lutsenko S, David LL, Burkhead JL. A systems approach implicates nuclear receptor targeting in the Atp7b(-/-) mouse model of Wilson's disease. Metallomics. 2012 Jul;4(7):660-8. doi: 10.1039/c2mt20017a. Epub 2012 May 8. PubMed [PMID: 22565294]
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