Research Overview
Primary research focus is in signal transduction in innate immunity. Specific areas of research focus on chemokine stimulation of primary human neutrophils and response of bronchial epithelium to tobacco smoke. Approaches include digital video microscopy methods, classical proteomics, genomics and both whole body and air-liquid interface exposure systems.
Current Research Projects
● Cellular Response to the Alaskan Smokeless Tobacco Mixture Iqmik and
Blueberry Extract
● Cellular Response to the Alaskan Smokeless Tobacco Mixture Iqmik
● Regulators of Rho in Lung Epithelial Cells Exposed to Cigarette Smoke
● In Vitro Modeling of Smoke Induced Loss of Lung Barrier Function
● Determining the Clinical Association of Immunologic memory and Tolerance in the
Natural Suppression of Chronic Hepatitis B Infection
Current Students
Graduate Students
2011-present Cindy Buffone
Masters’ student
Department of Biological Sciences
University of Alaska Anchorage
2011-present Cynthia Channell
Post-baccalaureate research
University of Alaska Anchorage
2011-present Jo Pak
Post-baccalaureate research
University of Alaska Anchorage
Undergraduate Students
2010-present Gaelen Dwyer
Student Research Assistant Alaska INBRE
University of Alaska Anchorage
2010-present Meagan Bryne
Alaska INBRE Undergraduate Fellowship
Undergraduate Research & NCMR Fellowship
University of Alaska Anchorage
2010-present Hazel Brogdon
NIH-NIDDK ANSIP
Marietta College, Marietta, OH
Publications (in chronological order)
Refereed Publications, Peer Reviewed (articles cited more than five times are so noted)
1. Siliciano, R.F., Lawton, T., Knall, C., Karr, R.W., Berman, P., Gregory, T. & Reinherz, E.L. 1988. Analysis of host-virus interactions in AIDS with anti-gp120 T cell clones: Effect of HIV sequence variation and a mechanism for CD4 + depletion. Cell 54:561-575. Times Cited 381. [PMID: 2969774]
2. Siliciano, R.F., Knall, C., Lawton, T., Berman, P., Gregory, T. & Reinherz, E.L. 1989. Recognition of HIV glycoprotein gp120 by T cells, role of monocyte CD4 in the presentation of gp120. J. Immunol. 142:1506-1511. Times Cited 28.
3. Ingold, A.L., Landel, C., Knall, C., Evans, G.A. & Potter, T.A. 1991. Co-engagement of CD8 with the T cell receptor is required for negative selection. Nature 352:721-723. Times Cited 82. [PMID: 1908563]
4. Knall, C., Ingold, A.L. & Potter, T.A. 1994. Analysis of co-receptor versus accessory molecule function of CD8 as a correlate of exogenous peptide concentration. Mol. Immunol. 31:875-883. Times Cited 7. [PMID: 8065371]
5. Knall, C., Smith, P.A. & Potter, T.A. 1995. CD8 dependent CTL require coengagement of CD8 and the TCR for phosphatidylinositol hydrolysis, but CD8 independent CTL do not and can kill in the absence of phosphatidylinositol hydrolysis. Internatl. Immunol. 7:995-1004. Times Cited 8. [PMID: 7577808]
6. Knall, C., Worthen, G.S., Buhl, A.M. & Johnson, G.L. 1995. IL-8 signal transduction in human neutrophils. Annals of the New York Academy of Sciences 277:288-291.
7. Knall, C., Young, S., Nick, J., Buhl, A.M., Worthen, G.S. & Johnson, G.L. 1996. IL-8 regulation of the Ras/Raf/MAP kinase pathway in human neutrophils. J. Biol. Chem. 271:2832-2838. Times Cited 158.http://www.jbc.org/cgi/reprint/271/5/2832
8. Nick, J.A., Avdi, N.J., Young, S.K., Knall, C., Gerwins, P., Johnson, G.L. & Worthen, G.S. 1997. Common and distinct intracellular signaling pathways in human neutrophils utilized by platelet activating factor and FMLP. J. Clin. Invest. 99:975-986. Times Cited 224]
9. Knall, C., Worthen, G.S. & Johnson, G.L. 1997. Interleukin 8 stimulated phosphatidylinositol-3-kinase activity regulates the migration of human neutrophils independent of extracellular signal-regulated kinase and p38 mitogen-activated protein kinases. Proc. Natl. Acad. Sci. USA. 94:3052-3057. Times Cited 191. [PMCID: PMC20320]
10. Jarpe, M.B., Knall, C., Mitchell, F., Buhl, A.M. & Johnson, G.L. 1998. [D-Arg1, D-Phe5, D-Trp7,9, Leu11] Substance P acts as a biased agonist towards neuropeptide and chemokine receptors. J. Biol. Chem. 273:3097-3104. Times Cited 45.
11. McLeish, K.R., Knall, C., Ward, R.A., Gerwins, P., Coxon, P.Y., Klein, J.B. & Johnson, G.L. 1998. Activation of mitogen-activated protein kinase cascades during priming of human neutrophils by Tumor Necrosis Factor- and GM-CSF. J. Leuk. Biol. 64:537-545. Times Cited 103.
http://www.jleukbio.org/cgi/reprint/64/4/537
12. Ambruso, D.R., Knall, C., Abell, A.N., Panepinto, J., Kurkchubasche, A., Thurman, G., Gonzalez-Aller, C., de Boer, M., Harbeck, R.J., Oyer, R., Johnson, G.L. & Roos, D. 2000. Human neutrophil immunodeficiency syndrome associated with an inhibitory Rac2 mutation. Proc. Natl. Acad. Sci. USA. 97:4654-4659. Times Cited 168. [PMC18288]
13. Anderson, M.S.*, Knall, C.*, Thurman, G., Mann, D., Cuack, N., Johnson, G.L & Ambruso, D.R. 2004. CP-64131, an aminobenzazepine with cytokine-like properties, stimulates human neutrophil functions through the p38-MAPK pathway. *These authors contributed equally to this work. J. Leuk. Biol. 76:477-483.
http://www.jleukbio.org/cgi/reprint/76/2/477
14. Seagrave, J., Knall, C., McDonald, J.D. & Mauderly, J.L. 2004. Diesel particulate material binds and concentrates a proinflammatory cytokine that causes neutrophil migration. Inhal. Toxicol. 16 Suppl 1:93-98. Times Cited 21. [PMID: 15204797]
15. Razani-Boroujerdi, S., Singh, S.P., Knall, C., Hahn, F.F., Philippides, J.C., Kalra, R., Langley, R.J. & Sopori, M. 2004. Chronic nicotine inhibits inflammation and promotes influenza infection. Cell. Immunol. 230:1-9. Times Cited 21. [PMID: 15541713]
16. Olivera, D.S., Boggs, S.E., Beenhouwer, C., Aden, J. & Knall, C. 2007. Cellular Mechanisms of Mainstream Cigarette Smoke-Induced Lung Epithelial Tight Junction Permeability Changes In Vitro. Inhal. Toxicol. 19:13–22. Times Cited 22. [PMID: 17127639]
17. Olivera, D., Knall, C., Boggs, S.E. & Seagrave, J. 2010. Cytoskeletal Modulation and Tyrosine Phosphorylation of Tight Junction Proteins Are Associated with Mainstream Cigarette Smoke Induced Permeability of Airway Epithelium. Experimental and Toxicologic Pathology 62:133–143.[PMID: 19376691]
